Outline Pasteur's experiment, including his methodology and findings. Explain the relationship between pathogens and decay.

Louis Pasteur (December 27 1822 - September 28, 1895) was a French chemist and microbiologist best known for his remarkable breakthroughs in the causes and prevention of disease. His experiments confirmed the germ theory of disease, also reducing mortality from puerperal (childbed) fever, and he created the first vaccine for rabies.

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Pasteur's Experiment

Where do the microbes come from to cause broth to decay?
Hypothesis: Microbes come from cells of organisms on dust particles in the air; not the air itself.
Method: Get multiple flasks one normally shaped as the control and the rest S shaped. the reason for the special shapes was to ensure no microbes can come from the air.
Each flask was boiled and placed at various locationsLeave for a few hours


Notice the dust collected on the entrances to the flaskshowing whether or not any microbes could enter. If there is any dust particles then microbes could have come from the air.


If any organisms are found in flasks with no dust particles then the organisms could not have come from the outside. This provides evidence against the spontanous generation theory and supports the germ theory.

Results/Conclusion: Organisms cannot enter the flasks in the above example therefore must come from germs inside the flask to cause decay